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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
美国
- 亚型:
50assays
- 保存条件:
-20C
- 克隆性:
多克隆
- 浓度:
Phototope-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
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文献和实验Western Blot Detection of Adrenergic Receptors
antibody, washed, and the antibody–antigen complexes are tagged with a secondary anti-IgG antibody coupled either to horseradish peroxidase (HRP) or alkaline phosphatase (AP) (1 ,7 ).
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
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