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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
美国
- 亚型：
100µl
- 形态：
W IP
- 保存条件：
-20C
- 克隆性：
多克隆
- 浓度：
GβL (86B8) Rabbit mAb
- 抗体名：
H M R Mk
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文献和实验7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
Analysis and Purification of Antibody Fragments Using Protein A, Protein G, and Protein L
media additives, DNA and endotoxins have to be removed from the mAb preparation to allow the proteins to be safely applied for human therapy. Moreover, antibody aggregates, clipped and low molecular weight species should also be removed.
needles. Binding Buffer (BioRad): make 1L. 314g/L ddH2 O and filter through 0.22 um, filter. pH=9.0 Elution buffer (BioRad): make 500 ml. 11g/500 ml ddH2 O and filter as before. pH=3.0 Regeneration buffer (BioRad): BioRad Affi-gel
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