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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃,避光
- 保质期:
6个月
- 库存:
1000
- 供应商:
南京森贝伽生物科技有限公司
DAPI染色液(10ug/ml)产品简介:
货号:SBJ-0221a
英文名称:DAPI staining solution (10ug/ml)
产品规格:10ml/50ml
保存条件:-20℃,避光,6个月
品牌:森贝伽
应用:细胞染色
DAPI染色液(10ug/ml)产品注意事项:
DAPI是一种能够与DNA强力结合的荧光染料,常用荧光显微镜观测,可以透过完整的细胞膜,可用于活细胞和固定细胞的染色,其工作浓度一般为0.3mmol/L或0.1ug/ml。显微镜下可以看到蓝色荧光的细胞,荧光显微镜观察细胞标记的效率高 ,且对活细胞无毒副作用。DAPI染色常用于细胞凋亡检测,染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常用于普通的细胞核染色以及某些特定情况下的双链DNA染色。DAPI的最大激发波长为340nm,最大发射波长为488nm, DAPI和双链DNA结合后,最大激发波长为360nm,最大发射波长为460nm。
DAPI染色液(10ug/ml)用途:
DAPI染色常用于细胞凋亡检测,染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常用于普通的细胞核染色以及某些特定情况下的双链DNA染色。
DAPI染色液(10ug/ml)其它产品:
SBJ-0221,DAPI染色液(10ug/ml),DAPI staining solution (5ug/ml),10ml/50ml,细胞染色,-20℃,避光,6个月
SBJ-0221a,DAPI染色液(10ug/ml),DAPI staining solution (10ug/ml),10ml/50ml,细胞染色,-20℃,避光,6个月
SBJ-0221c,DAPI染色液(1mg/ml),DAPI staining solution (1mg/ml),1ml,细胞染色,-20℃,避光,12个月
SBJ-1648,DAPI染色液(5ug/ml,含封片剂),DAPI staining solution (5ug/ml, sealed tablet),10ml,细胞染色,-20℃,避光,6个月
SBJ-1649,Hoechst33258染色液(含封片剂),Hoechst33258 staining solution (including sealed tablets),10ml,细胞染色,-20℃,避光,6个月
SBJ-0225a,Hoechst33258染色液(50×),Hoechst33258 staining solution (50 x),1ml,细胞染色,-20℃,避光,12个月
SBJ-0224,Hoechst33258染色液,Hoechst33258 staining solution,10ml,细胞染色,-20℃,避光,6个月
SBJ-0225,Hoechst33258染色液(1mg/ml),Hoechst33258 staining solution (1mg/ml),1ml,细胞染色,-20℃,避光,12个月
SBJ-0226,Hoechst33342染色液,Hoechst33342 staining solution,10ml,细胞染色,-20℃,避光,6个月
SBJ-0227,Hoechst33342染色液(1mg/ml),Hoechst33342 staining solution (1mg/ml),1ml,细胞染色,-20℃,避光,12个月
SBJ-1653,Hoechst33342染色液(含封片剂),Hoechst33342 staining solution (including sealed tablets),10ml,细胞染色,-20℃,避光,6个月
SBJ-0228,Hoechst33342/PI细胞凋亡染色试剂盒,Hoechst33342/PI cell apoptosis Staining Kit,100T,细胞染色,-20℃,避光,12个月
SBJ-0229,碘化丙啶PI染色液(50ug/ml),Propidium iodide PI staining (50ug/ml),10ml,细胞染色,-20℃,避光,6个月
SBJ-0230,碘化丙啶PI染色液(50ug/ml,含RNase),Propidium iodide PI staining solution (50ug/ml, containing RNase),10ml,细胞染色,-20℃,避光,6个月
SBJ-0231,碘化丙啶PI染色液(1mg/ml),Propidium iodide PI staining (1mg/ml),1ml,细胞染色,-20℃,避光,12个月
SBJ-0232,细胞周期与细胞凋亡检测试剂盒,Cell cycle and apoptosis assay kit,50T,细胞染色,-20℃,避光,12个月
SBJ-0233,细胞凋亡-Hoechst染色试剂盒,Hoechst staining kit for apoptosis,100T,细胞染色,-20℃,避光,12个月
SBJ-0234,吖啶橙染色液(1mg/ml),Acridine orange staining solution (1mg/ml),10ml,细胞染色,4℃,避光,12个月
SBJ-0234a,吖啶橙荧光染色试剂盒,Acridine orange fluorescent staining kit,100T,细胞染色,4℃,避光,6个月
SBJ-0236,吖啶橙/溴化乙锭双荧光染色试剂盒,Acridine orange / ethidium bromide fluorescence staining kit,100T,细胞染色,4℃,避光,6个月
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文献和实验DAPI Staining To visualize DNA, incubate fixed samples with 100 ng/ml 4',6'-diamidino-2-phenylindole hydrochloride (DAPI) in PBS for 30 min. Rinse 3x with PBTw. Materials PBS: Sambrook et al. (Molecular Cloning ). PBTw
化丙啶(propidium iodide,P1)高。 2.溶液配制(1)0.01mol/L PBS(pH7.0):取0.1mol/L NaH2 PO4 ・H2 O 34ml、0.1mol/LNa2 HPO4 66ml、NaCl 0.9g,溶于900m1双蒸水;(2)DAPI储存液:将0.5mgDAPI溶于5.0ml PBS中,分装,低温长期保存;(3)DAPI工作液:用PBS稀释DAPI储存液,终浓度为0.1ug/ml。 3.染色程序(1)培养的单层细胞(未固定)或新鲜组织的冰冻切片等,PBS漂洗5分钟
Cell Cycle Staining Protocol-DAPI
in .5-2.0ml DAPI stain solution (final concentration of 1x106 cells/ml) and incubate for 30 min at 4degrees or on ice. DAPI Stain Solution 0.1%TritonX 100 in 10ml PBS add 100ul of 1mg/ml DAPI to the 10ml TritonX solution
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