Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Rabbit, )
产品应用
ELISA=1:5000-10000 IHC-P=1:100-500 Flow-Cyt=1μg /test ICC=1:100 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量
132kDa
检测分子量
kDa
细胞定位
细胞膜
性 状
Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Ser636/639: PM(p-S)PK(p-S)VS
亚 型
IgG
纯化方法
affinity purified by Protein A
缓 冲 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
产品图片
paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-IRS1 (Ser636 + Ser639)) Polyclonal Antibody, Unconjugated (bs-3201R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.