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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Normal Human Small Airway Epithelial Cells
- 库存:
大量
- 供应商:
北京百奥创新科技有限公司
- 肿瘤类型:
详见说明书
- 细胞类型:
详见说明书
- 品系:
详见说明书
- 组织来源:
详见说明书
- 相关疾病:
详见说明书
- 物种来源:
详见说明书
- 免疫类型:
详见说明书
- 细胞形态:
详见说明书
- 是否是肿瘤细胞:
是
- 器官来源:
详见说明书
- 运输方式:
干冰运输
- 年限:
详见说明书
- 生长状态:
良好
- 规格:
1 vial
产品简介:Human Airway Epithelial Cells, when grown in LIBro Medium, provide an ideal serum-free culture model for the accurate studies of toxicity, cystic fibrosis, asthma, pathogenesis, pharmacology or airway wound healing. HSAEC are cultured without retinoic acid and cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our HSAEC are quality tested in LIBro SAE Medium to ensure optimal growth over a period of at least 15 population doublings at rates equal to or greater than other commercially available media. Cell Features: HBTEC are cryopreserved as primary cells. Cells are isolated from human bronchi/trachea and expanded once in culture vessels before being harvested for cryopreservation. HSAEC are cryopreserved after primary cells are isolated from human lung tissue and expanded once in culture vessels before being harvested for cryopreservation. Human Airway Epithelial Cells are not exposed to retinoic acid during isolation or expansion. Human Airway Epithelial Cells are extensively tested for quality and optimal performance. AcceGen Biotech guarantees performance and quality.
产品图片:
细胞培养流程:
1、培养前准备
(1)培养器具:推荐使用25 cm2培养瓶或60 mm培养皿(或更小)。
(2)培养基:准备适合培养该细胞的培养基(具体咨询北京百奥创新科技有限公司客服推荐培养基),建议用量5 mL(或更少)。
2、细胞解冻
将包装中的冻存管取出后立即放在不高于37℃的水中,并在2分钟内摇晃解冻。
3、细胞复苏
(1)取10ml培养基置于离心管中,用70%酒精或阳离子洗涤剂渗透纱布消毒冻存管,用消毒纱布包裹管身,拧开管盖后,将细胞悬浮液转移到离心管中;
(2)1000 rpm离心3分钟,弃掉上清液;
(3)将细胞重新悬浮在5ml上述培养基中(不清洗细胞)。在25 cm2的培养瓶或60 mm的培养皿中进行培养。
4、细胞传代
在传代之前,确保细胞增殖,因为过度稀释可能导致细胞死亡,尤其是血细胞和淋巴细胞。
5、细胞冻存
待细胞生长状态良好时,尽早进行细胞冻存。
北京百奥创新科技有限公司是中关村高新技术企业,于2017年在北京成立。百奥创新依托于中科院的科研技术力量,以中科院资深科学家为核心,整合经验丰富的商业团队及管理团队组建而成,专注于免疫学、细胞生物学、分子生物学 等领域核心试剂产品的研发、生产与销售。
百奥创新在产品自主研发的同时,也与全球多家生命科学试剂供应商建立良好的合作关系,基于“客户第一 ”的核心理念,向全国10000+客户提供200000+优质产品。公司以北京运营中心为主体,在天津、上海、浙江、江苏、广州、深圳、武汉、成都、重庆等地建立办事处及联络中心,并建有北京、天津、深圳三大专业化库存与物流基地,以更好地服务全国客户。
百奥创新将以品质树立品牌,以服务赢得口碑,致力于成长为重要的生物原料及生物化学试剂开发与成果转化的开拓者,并成为行业领先的优质供应商。
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文献和实验Culture of Normal Human Airway Epithelial Cells and Measurement of Mucin Synthesis and Secretion
The plasticity of conducting airway epithelia is well recognized (1 –3 ). Under normal conditions, the epithelia express mucociliary function, which is the first pulmonary defense mechanism against inhaled air pollutants. Aberrance
Human Airway Epithelial Cell Culture
Development of methods to culture airway epithelial cells has been needed to carry out research into various lung diseases, such as cancer, cystic fibrosis, and bronchial asthma. However, the culture of airway epithelial cells remained
Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells
One of the main functions of the airway mucosa is to maintain a mechanical barrier at the air–surface interface and to protect the respiratory tract from external injuries. Differentiation of human airway epithelial cells (hAECs) to polarized
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