The urothelial cells, the cells lining the surface of the urinary bladder, are comprised of a unique cell type with high plasticity and a variety of cell functions. They are the first line of bladder defense and serve as an interface between pathogens that is equipped with several defense mechanisms to prevent adherence of pathogens and maintain impermeability to urinary solutes. The Urothel Epithelial Cells express both estrogen receptor-alpha and beta, epidermal growth factor receptor and fibroblast growth factor receptor, these receptors play a major role on urothelial cell response to injury and infection. They also release a number of cytokines and other immune system mediators and this newly recognized immunoregulatory potential of the urothelial cell warrants further investigation through the application of two recent developments: 1) the ability to culture normal urothelial cells in the laboratory and 2) the understanding of how immunoregulatory communications are generated for the biological functions of urothelial cells through signal transduction pathways.HUC from AcceGen Research Laboratories are isolated from the human bladder. HUC are cryopreserved at passage one and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HUC are characterized by immunofluorescent method with antibodies to cytokeratin-18, -19 and vimentin. HUC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUC are guaranteed to further expand for 15 population doublings in the condition provided by AcceGen Research Laboratories. 尿路上皮细胞,即膀胱表面的细胞,由具有高可塑性和多种细胞功能的独特细胞类型组成。它们是膀胱防御的第一道防线,充当病原体之间的界面,配备多种防御机制以防止病原体粘附并保持对尿溶质的不渗透性。尿路上皮细胞表达雌激素受体-α和β、表皮生长因子受体和成纤维细胞生长因子受体,这些受体在尿路上皮细胞对损伤和感染的反应中起主要作用。它们还释放许多细胞因子和其他免疫系统介质,这种新发现的尿路上皮细胞的免疫调节潜力值得通过应用两个最近的发展来进一步研究:1)在实验室培养正常尿路上皮细胞的能力和 2)理解免疫调节通讯是如何产生的 用于通过信号转导途径发挥尿路上皮细胞的生物学功能。来自 AcceGen 研究实验室的 HUC 从人类膀胱中分离出来。 HUC 在第 1 代冷冻保存并冷冻交付。 每个小瓶在 1 ml 体积中包含 >5×10^5 个细胞。 HUC 的特征是用针对细胞角蛋白-18、-19 和波形蛋白的抗体的免疫荧光方法。 HUC 对 HIV-1、HBV、HCV、支原体、细菌、酵母和真菌呈阴性。 HUC 保证在 AcceGen 研究实验室提供的条件下进一步扩大 15 倍人口。
Quality Control
All cells test negative for mycoplasma, bacteria, yeast, and fungi.