Tonsil epithelium is a heterogeneous lymphoid organ containing two different types of actively differentiating epithelia, the lining stratified squamous epithelium and the reticulated crypt, or lymphoepithelium. The tonsil crypts represent a specialized compartment, important in the immunological functions of the tonsil because of the immediate proximity of the epithelial and lymphoid tissues. In Situ staining of tonsil epithelium suggests that expression of the simple epithelial keratins K8, K18 and K19 is located to the crypt epithelial cells. Cell culture of tonsil epithelium produces heterogeneous squamous epithelial culture in which differentiation appears to be ongoing and keratin expression patterns are consistent with those found in tonsil epithelial cells in situ. HTonEpiC from AcceGen Research Laboratories are isolated from human normal tonsil tissue. HTonEpiC are cryopreserved at passage one culture and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HTonEpiC are characterized by immunofluorescent method with cytokeratine antibodies. HTonEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 扁桃体上皮是一种异质淋巴器官,包含两种不同类型的积极分化的上皮,内膜分层鳞状上皮和网状隐窝,或淋巴上皮。扁桃体隐窝是一个特殊的隔室,由于与上皮组织和淋巴组织紧邻,所以在扁桃体的免疫功能中非常重要。扁桃体上皮原位染色显示,单纯上皮角蛋白K8、K18和K19的表达位于隐窝上皮细胞。扁桃体上皮细胞培养产生异质鳞状上皮细胞,其中分化似乎正在进行,角蛋白表达模式与原位扁桃体上皮细胞中发现的一致。来自AcceGen研究实验室的HTonEpiC是从人体正常扁桃体组织中分离出来的。HTonEpiC在第1代培养时冷冻保存,冷冻交付。每个小瓶含有1ml体积的>5×10^5细胞。HTonEpiC采用细胞角蛋白抗体免疫荧光法进行表征。HTonEpiC对HIV-1、HBV、HCV、支原体、细菌、酵母和真菌均为阴性。
Quality Control
All cells test negative for mycoplasma, bacteria, yeast, and fungi.