The ciliary epithelium rests upon the folds of the ciliary processes. It consists of two layers of cells which appear structurally and functionally distinct. The basal membranes of the pigmented ciliary epithelial cells face the stroma, and the basal membranes of the non-pigmented ciliary epithelial cells (NPCEC) abut the aqueous humor. The NPCEC is largely responsible for the formation of aqueous humor in the mammalian eye. They transfer solute from the stroma of the ciliary processes into the posterior chamber. The resulting osmotic gradient drives water from the stroma into the aqueous humor. It is only the NPCEC cells which form a complete permeability barrier, since they display tight junctions linking adjoining cells.HNPCEC from AcceGen Research Laboratories are isolated from human ciliary bodies. HNPCEC are cryopreserved at primary culture and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HNPCEC are characterized by immunofluorescent method with antibodies to cytokeratin-18, cytokeratin-19. HNPCEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 纤毛上皮位于纤毛突的褶皱上。它由两层细胞组成,这两层细胞在结构和功能上截然不同。着色纤毛上皮细胞的基膜面向基质,非着色纤毛上皮细胞的基膜紧邻房水。在哺乳动物的眼睛中,NPCEC主要负责房水的形成。它们将溶质从睫突间质转移到后房。由此产生的渗透梯度促使水从基质进入房水。只有非亲细胞形成完整的通透性屏障,因为它们显示紧密连接相邻细胞。来自AcceGen研究实验室的HNPCEC是从人体纤体中分离出来的。HNPCEC原代培养低温保存,冷冻交付。每个小瓶含有1ml体积的>5×10^5细胞。用细胞角蛋白18、细胞角蛋白19抗体免疫荧光法对HNPCEC进行了表征。HNPCEC对HIV-1、HBV、HCV、支原体、细菌、酵母和真菌均为阴性。
Quality Control
All cells test negative for mycoplasma, bacteria, yeast, and fungi.