AcceGen人单核细胞白血病细胞THP-1细胞(ABC-TC1234)
产品简介:
产品英文名称:THP-1;
货号:ABC-TC1234;
物种:人类;
产品类型:人类白血病细胞系;
描述:细胞具有吞噬功能(乳胶珠和致敏红细胞),缺乏表面和细胞质免疫球蛋白。佛波醇酯12-O-十四烷酰基佛波醇-13-乙酸酯(TPA)可诱导单核细胞分化;
生长特征:单核细胞;
生物安全等级:一级;
运输方式:干冰运输;
储存方式:液氮储存;
应用:仅供研究使用。
THP-1 is a monocyte cell line derived from the peripheral blood of an acute monocytic leukemia patient. These cells possess Fc and C3b receptors but lack surface or cytoplasmic immunoglobulins. Notably, the HLA haplotypes of THP-1 are HLA-A2, -A9, -B5, -DRW1, and -DRW2. The monocytic nature of THP-1 cells is evident through various characteristics, including the presence of α-naphthyl butyrate esterase activities that are susceptible to NaF inhibition, lysozyme production, phagocytosis capability for latex particles and sensitized sheep erythrocytes, and the ability to restore T-lymphocyte response to Con A.
Why choose THP-1 from AcceGen?
THP-1 cells boast high viability and quality, maintained under sterile, mycoplasma-free conditions with optimal incubation. They are cryopreserved by professional operators and undergo rigorous quality control, ensuring their reliability and suitability for various research applications.
FOR RESEARCH USE ONLY
THP-1 cells find diverse applications due to their unique characteristics. They serve as an ideal transfection host, facilitating genetic studies and manipulation. Additionally, these cells offer valuable insights into monocyte biology and immune responses, acting as a multifaceted model for studying monocyte-macrophage differentiation. Moreover, THP-1 cells prove invaluable in investigating the immune-modulating effects of compounds under both activated and resting conditions, making them essential in drug development and immunological research.
Growth Conditions |
37 ℃, 5% CO2 |
Cell Type |
Monocyte |
Growth Mode |
Suspension |
Product Type |
Human Leukemia Cell Lines |
细胞培养:
1、培养前准备
(1)培养器具:推荐使用25 cm2培养瓶或60 mm培养皿(或更小)。
(2)培养基:准备适合培养AcceGen人单核细胞白血病细胞THP-1细胞的培养基,建议用量5 mL(或更少)。
2、细胞解冻
将包装中的冻存管取出后立即放在不高于37℃的水中,并在2分钟内摇晃解冻。
3、细胞复苏
(1)取10ml培养基置于离心管中,用70%酒精或阳离子洗涤剂渗透纱布消毒冻存管,用消毒纱布包裹管身,拧开管盖后,将细胞悬浮液转移到离心管中;
(2)1000 rpm离心3分钟,弃掉上清液;
(3)将细胞重新悬浮在5ml上述培养基中(不清洗细胞)。在25 cm2的培养瓶或60 mm的培养皿中进行培养。
4、细胞传代
在传代之前,确保细胞增殖,因为过度稀释可能导致细胞死亡,尤其是血细胞和淋巴细胞。
5、细胞冻存
待AcceGen人单核细胞白血病细胞THP-1细胞生长状态良好时,尽早进行细胞冻存。
产品信息:
货号 |
产品名称 |
规格 |
ABC-TC1234 |
THP-1 |
1 vial |