中文名称:NUGC-4/人胃癌细胞
英文名称:NUGC-4 cell
品牌:accegen
产品简介:印戒细胞癌。NUGC-4/人胃癌细胞
产品类型:人膀胱癌症细胞系
NUGC-4, also referred to as ‘Nagoya University-Gastric Cancer-4’, ‘NU-GC-4’, and ‘NUGC4’, is a human gastric cancer cell line established from the patient during surgery. This cell line was derived from signet ring cell carcinoma of human stomach at the metastatic paragastric lymph nodes. When cultured in vitro, NUGC-4 cells exhibit a spherical epithelial-like morphology and display characteristics of cell adhension, although a few free-floating cells may be also present. On average, the doubling time of NUGC-4 is about 30 hours, and it should be cultured in PRMI1640 with 10% FBS.
Why choose NUGC-4 from AcceGen?
AcceGen supports NUGC-4 cells at more than 5×105 cells in 1mL volume with professional packaging of cryopreservation to ensure high quality and viability. Accegen NUGC-4 is negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. With the condition provided by AcceGen, the population doubling of NUGC-4 can reach more than 15 times.
FOR RESEARCH USE ONLY
As a cancer cell line, NUGC-4 is widely used in scientific research, such as searching for key factors of the formation of human gastric cancer and targets for anti-cancer drugs. Epidermal growth factor (EGF) strongly activates the tyrosine phosphorylation in NUGC-4. Phosphatidylinositol 3-kinase (PI 3-kinase), which is mainly activated by tyrosine kinases, was precipitated with anti-phosphotyrosine antibodies in NUGC-4. Exogenous hepatocyte growth factor can stimulate the proliferation of NUGC-4, and MET tyrosine kinase inhibitors inhibited this proliferation of NUGC-4. Investigation of NUGC-4 cell line and the interactions with growth factors may provide the direction for identifying the signaling pathway of gastric cancer and screening of potential therapeutic targets.
细胞培养流程:
1、培养前准备
(1)培养器具:推荐使用25 cm2培养瓶或60 mm培养皿(或更小)。
(2)培养基:准备适合培养NUGC-4/人胃癌细胞的培养基(具体咨询北京百奥创新科技有限公司客服推荐培养基),建议用量5 mL(或更少)。
2、细胞解冻
将包装中的冻存管取出后立即放在不高于37℃的水中,并在2分钟内摇晃解冻。
3、细胞复苏
(1)取10ml培养基置于离心管中,用70%酒精或阳离子洗涤剂渗透纱布消毒冻存管,用消毒纱布包裹管身,拧开管盖后,将细胞悬浮液转移到离心管中;
(2)1000 rpm离心3分钟,弃掉上清液;
(3)将细胞重新悬浮在5ml上述培养基中(不清洗细胞)。在25 cm2的培养瓶或60 mm的培养皿中进行培养。
4、细胞传代
在传代之前,确保细胞增殖,因为过度稀释可能导致细胞死亡,尤其是血细胞和淋巴细胞。
5、细胞冻存
待NUGC-4/人胃癌细胞生长状态良好时,尽早进行细胞冻存。
Product Name |
NUGC-4/人胃癌细胞 |
Cat.No |
ABC-TC0862 |
Species |
Human |
Size/Quantity |
1 vial |
Biosafety Level |
1 |
Shipping Info |
Dry Ice |
Storage |
Liquid Nitrogen |
Description |
Signet ring cell carcinoma. |
Growth Properties |
Spherical |
Application |
For research use only |
Product Type |
Human Gastric Cancer Cell Lines |
Product Image |
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