Rat Retinal Microvascular Endothelial Cells from AcceGen are isolated from tissue of 5-day-old neonatal Sprague-Dawley Rats. Rat Retinal Microvascular Endothelial Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen’s Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and are delivered frozen. Rat Retinal Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications. 从5日龄新生sd大鼠组织中分离出AcceGen视网膜微血管内皮细胞。大鼠视网膜微血管内皮细胞在涂有明胶溶液的T75组织培养瓶中培养0.5小时,在AcceGen细胞培养基中培养3-7天。文化被扩展。在运输之前,细胞从烧瓶中分离出来,并立即冷冻保存在小瓶中。每个小瓶至少包含1×10^6细胞每毫升,并提供冷冻。 大鼠视网膜微血管内皮细胞可用于细胞间粘附、迁移、血管形成或跨内皮阻力(TER)的检测。对内皮细胞培养进行的标准生化程序包括PCR、Western blotting、免疫沉淀、免疫荧光染色或免疫荧光流式细胞术,或生成用于所需研究应用的细胞衍生物。
Quality Control
All cells test negative for mycoplasma, bacteria, yeast, and fungi.