EGFR 单克隆抗体

Western Blot（WB）已验证样本:Hela
免疫荧光（IF）已验证样本: Hela

WB 1:1000-3000, IF 1:100-200

Monoclonal

IgG

Synthetic Peptide

EGFR

Avian erythroblastic leukemia viral (v erb b) oncogene homolog;Cell growth inhibiting protein 40;Cell proliferation inducing protein 61;EGF R;EGFR;EGFR;Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog);Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian);Epidermal growth factor receptor;erb-b2 receptor tyrosine kinase 1;ERBB;ERBB1;Errp;HER1;mENA;NISBD2;Oncogen ERBB;PIG61;Proto-oncogene c-ErbB-1;Receptor tyrosine protein kinase ErbB 1;Receptor tyrosine-protein kinase ErbB-1;SA7;Species antigen 7;Urogastrone;v-erb-b Avian erythroblastic leukemia viral oncogen homolog;wa2;Wa5抗体

P00533

134kDa

170kDa

Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).

Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.

1 mg/mL

在-20℃下储存有效期为12个月。 避免冻融循环。

PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Protein A purification

Cancer; Signal Transduction

Clone:2B3

Unconjugated

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.Isoform 2 may act as an antagonist of EGF action.