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文献和实验E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
with occasional mixing. Avoid vigorous mixing as this will shear chromosomal DNA and lower plasmid purity. (Store Solution II tightly capped when not in use.) 4. Add 250 ul ice-cold Buffer N3 and mix gently but throughly by inverting tube several times
E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
medium and discard. To the bacterial pellet add 250 u Solution I/RNase A. Resuspend cells completely by vortexing or pipetting up and down. Complete resuspension of cell pellet is vital for obtaining good yields. 4. Add 250 u Solution II and mix
and thereby ensure maximal growth and plasmid DNA yields). 2nd Day -DNA extraction and purification Materials required QIAGEN Mini-prep kit Microfuge Cell Lysis/DNA Extraction 1. Concentrate the bacteria by transferring 1 ml bacterial suspension
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