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文献和实验Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins
) Source 15Q anion‐exchange column (GE Healthcare) Vivaspin 20 ultrafiltration spin column (Sartorius Stedim Biotech) with 10‐kDa molecular weight (MW) cutoff
Site-directed hydroxy radical mapping of nucleosome positions in vitro
by the hydroxyl radicals, DNA scission does not proceed to completion. Typically 5-20% of the nucleosomes will be mapped. The advantage of the high specificity of the radical attack on DNA is that it results in scission of the DNA backbone at a single site
Chromatin Assembly Using Drosophila Systems
and appropriate tubes 12,000 to 15,000 and 3,500 MWCO dialysis tubing FPLC apparatus
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