Vivaspin 20, 3 kDa MWCO PES

蛋白浓缩，超滤离心管选择指南

保证高截留，又不会牺牲流速。 管盖颜色区分MWCO：不同分子量对应不同颜色，一秒钟判断是否拿错管子。 2.选对上样体积，覆盖不同制备方案 赛米科超滤离心管分为两种容量，均采用低吸附PES膜，蛋白回收率≥90%： 15mL系列：可处理起始体积10-14mL的样品，快速获得高浓度蛋白，用于后续晶体筛选或动物实验。作为实验室最常用规格，适合细胞培养上清、裂解液的日常浓缩与缓冲液置换。 4mL系列：小量样品更合适的选择，效率更高，浓缩更快。 每个系列均配备刻度标识和书写区，离心后直接读数，不必反复开盖取样

Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins

) Source 15Q anion‐exchange column (GE Healthcare) Vivaspin 20 ultrafiltration spin column (Sartorius Stedim Biotech) with 10‐kDa molecular weight (MW) cutoff

Site-directed hydroxy radical mapping of nucleosome positions in vitro

by the hydroxyl radicals, DNA scission does not proceed to completion. Typically 5-20% of the nucleosomes will be mapped. The advantage of the high specificity of the radical attack on DNA is that it results in scission of the DNA backbone at a single site