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文献和实验labelling study might be conducted in a plant, so as to measure over short time nitrogen and sulfur acquisition, and assimilates arising from a labelled source.
for this protocol.）2. Wash the oligo dT cellulose 3X with 750 l 1X NETS. （Spin down gently between washes and aspirate off the wash.）3. Aspirate off final 1X NETS wash and resuspend the cellulose in 750 l 2X NETS.4. Incubate the RNA at 65 !C for 10 minutes.5. Add
Phosphoamino acid analysis:Mark Kamps's method
or enough to submerge your piece of membrane. 3.Incubate in 110℃ oven for 60 min. 4.Microfuge sample full speed for at least 1 min. 5.Transfer supernatant to new tube and lyophilize on Speedivac.(It takes about 3 hr.) 6.Resuspend in H2O,and microfuge 5 min.Spotting
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