GEL FILTRATION CAL KIT HMW

Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron Microscopy

are from the Siegel�Monte paper (indicated S-M under source), or the CRC Handbook of Biochemistry (3 ) (indicated HBC). They agree with the values listed for R s in the GE Healthcare gel filtration calibration kit, with the exception of ferritin. The “M r calc

设计保护碱基参考资料

as indicated in the DNA Extraction Kit (#K0513) followed by ethanol precipitation or gel filtration. DNA (0.5µg) and 10u of REase were incubated for 1 hour at the recommended incubation temperature and optimal buffer for each enzyme in a total volume of 40µl

Preparation of probes for in situ hybridization

Unreacted dye is then removed by either two rounds of ethanol precipitation and washing (carrier tRNA can be added if needed), or by gel filtration through G50 columns. Specific activity of the probes is calculated by absorption spectroscopy. NB