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文献和实验Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
1) Perform agarose gel/ethidium bromide electrophoresis to fractionate DNA fragments. Any type or grade of agarose may be used. It is strongly recommended however, that fresh TAE buffer or TBE buffer be used as running buffer. Do not reuse running buffer
Establishment and Maintenance of Normal Human Keratinocyte Cultures
Keratinocytes are the major cell type of the epidermis, which is the stratified squamous epithelium forming the outermost layer and thus providing the barrier function of the skin. The keratinocytes lie on a highly specialized extracellular
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