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- BD biosciences
- 558455.0
- 美国
- 2025年07月13日
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上海恪敏生物科技有限公司
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文献和实验Western Blotting with Horseradish Pe
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL
or 4°C; Wash 3 times in 1X PBS for 5 minutes each; Permeabilize with freshly made 1X PBS/0.5% Triton X-100 (containing the RNAse-inhibitor, 2mM Vanadyl Ribonucleoside Complex, in case of a subsequent RNA-FISH) on ice for 3.5-5 minutes
_blank()) + geom_hline(aes(yintercept = 0), data1,alpha=0.2) #设置主题+ labs(fill="Fold change") +ylab("Picture of text") #坐标标题+scale_x_discrete(breaks = c("A","B","C","D","E","F"),labels = c("D0","D1,"D2,"D3,"D4,"D5)) #更换刻度文本+ expand_limits(y=-300) #扩大
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