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货号:554724
名称:PROTEIN TRANSPORT INHIBITOR SOLN 0.7ML
品牌:BD biosciences
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文献和实验, and the unexpected location of the inhibitor-binding site suggests a transport mechanism for arachidonic acid and provides functional insights into leukotriene biosynthesis. This chapter describes how this human integral membrane crystal structure was solved
Metabolic labeling Immunoprecipitation
/ml Cysteine-HCl,100µg/ml methionine,sterile filtered • Tris-buffered saline (TBS): 20mM Tris,150mM NaCl,pH 7.4 • X1000 Protease inhibitor soln: 500mM PMSF (Sigma P-7626)in EtOH,10mg/ml leupeptin (Sigma L-2023)in water,10mg/ml pepstatin A (P-4265
Metabolic labeling Immunoprecipitation
) supplemented w/ 10% dialyzed FCS, & Gln • Chase Medium: Labeling medium (above) + 500µg/ml Cysteine-HCl, 100µg/ml methionine, sterile filtered • Tris-buffered saline (TBS): 20mM Tris, 150mM NaCl, pH 7.4 • X1000 Protease inhibitor soln: 500mM PMSF (Sigma
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