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InVivoMAb anti-mouse CD28
InVivoMAb anti-mouse CD28
请参考说明
in vivo T cell stimulation/activation ;in vitro T cell stimulation/activation
Mouse
欣博盛生物科技有限公司
大量
请参考说明
Mouse
单克隆
4°C
液体
Mouse IgG1
A20 cells expressing mouse CD28 and a recombinant mouse CD28-Ig fusion protein
1MG,5MG,25MG,50MG,100MG
InVivoMAb anti-mouse CD28单克隆抗体,货号:BE0328 ,有五种规格可供选择!
研发背景
D665单克隆抗体可与小鼠CD28反应。CD28是T细胞中关键的共刺激受体,同时也是Ig超家族成员之一,其主要由胸腺细胞、大部分外周血T细胞和NK细胞表达。CD28是CD80 (B7-1)和CD86 (B7-2)的受体。通过CD28信号传导可诱导IL-2和IL-2受体表达和T细胞增殖。D665抗体是一种CD28超激动剂,在多种小鼠疾病模型中常用来诱导Treg细胞在体内的扩增。
产品信息
克隆号(Clone) |
D665 |
同种型(Isotype) |
Mouse IgG1 |
免疫原(Immunogen) |
A20 cells expressing mouse CD28 and a recombinant mouse CD28-Ig fusion protein |
应用(Reported Applications) |
in vivo T cell stimulation/activation in vitro T cell stimulation/activation |
形式(Formulation) |
PBS, pH 7.0 Contains no stabilizers or preservatives |
内毒素(Endotoxin) |
<2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay |
纯度(Purity) |
>95% Determined by SDS-PAGE |
无菌(Sterility) |
0.2 μM filtered |
纯化(Purification) |
Protein G |
分子量(Molecular Weight) |
150 kDa |
储存(Storage) |
The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
订购详情
产品名称 |
货号 |
规格 |
InVivoMAb anti-mouse CD28 |
BE0328 |
1MG,5MG,25MG,50MG,100MG |
参考文献
Schmidt, T., et al. (2016). 'Induction of T regulatory cells by the superagonistic anti-CD28 antibody D665 leads to decreased pathogenic IgG autoantibodies against desmoglein 3 in a HLA-transgenic mouse model of pemphigus vulgaris.' Exp Dermatol 25(4): 293-298.
Win, S. J., et al. (2016). 'In vivo activation of Treg cells with a CD28 superagonist prevents and ameliorates chronic destructive arthritis in mice.' Eur J Immunol 46(5): 1193-1202.
Schuhmann, M. K., et al. (2015). 'CD28 superagonist-mediated boost of regulatory T cells increases thrombo-inflammation and ischemic neurodegeneration during the acute phase of experimental stroke.' J Cereb Blood Flow Metab 35(1): 6-10.
Dennehy, K. M., et al. (2006). 'Cutting edge: monovalency of CD28 maintains the antigen dependence of T cell costimulatory responses.' J Immunol 176(10): 5725-5729.
详情请咨询 BioXcell 中国授权代理-欣博盛生物科技
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Although regulatory T (Treg) cells are necessary to prevent autoimmune diseases, including arthritis, whether Treg cells can ameliorate established inflammatory disease is controversial. Using the glucose-6-phosphate isomerase (G6PI)-induced arthritis model in mice, we aimed to determine the therapeutic efficacy of increasing Treg cell number and function during chronic destructive arthritis. Chronic destructive arthritis was induced by transient depletion of Treg cells prior to immunization with G6PI. At different time points after disease induction, mice were treated with a CD28 superagonistic antibody (CD28SA). CD28SA treatment during the induction phase of arthritis ameliorated the acute signs of arthritis and completely prevented the development of chronic destructive arthritis. CD28SA treatment of mice with fully developed arthritis induced a significant reduction in clinical and histological signs of arthritis. When given during the chronic destructive phase of arthritis, 56 days after disease induction, CD28SA treatment resulted in a modest reduction of clinical signs of arthritis and a reduction in histopathological signs of joint inflammation. Our data show that increasing the number and activation of Treg cells by a CD28SA is therapeutically effective in experimental arthritis.
Pemphigus vulgaris (PV) is a potentially life-threatening autoimmune disease of the skin and mucous membranes. Its pathogenesis is based on IgG autoantibodies that target the desmosomal cadherins, desmoglein 3 (Dsg3) and desmoglein 1 (Dsg1) and induce intra-epidermal loss of adhesion. Although the PV pathogenesis is well-understood, therapeutic options are still limited to immunosuppressive drugs, particularly corticosteroids, which are associated with significant side effects. Dsg3-reactive T regulatory cells (Treg) have been previously identified in PV and healthy carriers of PV-associated HLA class II alleles. Ex vivo, Dsg3-specific Treg cells down-regulated the activation of pathogenic Dsg3-specific T-helper (Th) 2 cells. In this study, in a HLA-DRB1*04:02 transgenic mouse model of PV, peripheral Treg cells were modulated by the use of Treg-depleting or expanding monoclonal antibodies, respectively. Our findings show that, in vivo, although not statistically significant, Treg cells exert a clear down-regulatory effect on the Dsg3-driven T-cell response and, accordingly, the formation of Dsg3-specific IgG antibodies. These observations confirm the powerful immune regulatory functions of Treg cells and identify Treg cells as potential therapeutic modulators in PV.
While the detrimental role of non-regulatory T cells in ischemic stroke is meanwhile unequivocally recognized, there are controversies about the properties of regulatory T cells (Treg). The aim of this study was to elucidate the role of Treg by applying superagonistic anti-CD28 antibody expansion of Treg. Stroke outcome, thrombus formation, and brain-infiltrating cells were determined on day 1 after transient middle cerebral artery occlusion. Antibody-mediated expansion of Treg enhanced stroke size and worsened functional outcome. Mechanistically, Treg increased thrombus formation in the cerebral microvasculature. These findings confirm that Treg promote thrombo-inflammatory lesion growth during the acute stage of ischemic stroke.
CD28 and CTLA-4 are the major costimulatory receptors on naive T cells. But it is not clear why CD28 is monovalent whereas CTLA-4 is bivalent for their shared ligands CD80/86. We generated bivalent CD28 constructs by fusing the extracellular domains of CTLA-4 or CD80 with the intracellular domains of CD28. Bivalent or monovalent CD28 constructs were ligated with recombinant ligands with or without TCR coligation. Monovalent CD28 ligation did not induce responses unless the TCR was coligated. By contrast, bivalent CD28 ligation induced responses in the absence of TCR engagement. To extend these findings to primary cells, we used novel superagonistic and conventional CD28 Abs. Superagonistic Ab D665, but not conventional Ab E18, predominantly ligates CD28 bivalently at low CD28/Ab ratios and induces Ag-independent T cell proliferation. Monovalency of CD28 for its natural ligands is thus essential to provide costimulation without inducing responses in the absence of TCR engagement.