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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 保存条件:
-20℃
- 克隆性:
Polyclonal
- 适应物种:
Human,
- 宿主:
Rabbit
- 应用范围:
WB,
- 浓度:
1mg/1ml
- 抗体英文名:
Mouse Irgm1 Antibody (C-term) (Ascites)
- 抗体名:
Mouse Irgm1 Antibody (C-term) (Ascites)
- 规格:
500ul
一抗种属的来源
多克隆抗体存在于免疫动物的血清中,可以从血清中纯化获得。多克隆抗体能识别多个抗原位点,可用于多种免疫学反应,并且制备时间短,成本低,因此被广泛应用于免疫学研究领域和免疫学诊断领域。多抗制备流程:
抗体纯化包括从血清(多克隆抗体)、腹水液或者杂交瘤细胞系(单克隆抗体)的培养物上清液中分离出抗体。
纯化方法从非常粗制到高度特异性不等:
粗制——血清总蛋白(包括免疫球蛋白)的一个子集形成沉淀
一般——对特定抗体类别(如 IgG)进行亲和纯化,但不考虑抗原特异性
特异性——仅对在样品中与特定抗原分子结合的那些抗体进行亲和纯化
获得可用抗体所需的纯化水平取决于该抗体的预期用途。
我们提供种类繁多的琼脂糖珠、亲和柱和纯化试剂盒,以从血清、腹水液或组织培养上清液中纯化全部或特异性抗体。 我们提供的产品包括多种蛋白 A、蛋白 G、蛋白 A/G 和蛋白 L。
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文献和实验Purification of Antiserum or Ascites by Protein A/G Chromatography
A and protein G bind about 20 mg of IgG per ml of conjugated agarose. Therefore, the binding capacity of a 10 ml (5 ml) column is 200 mg (100 mg) of IgG. A high-titer rabbit antiserum has roughly 5 mg/ml of IgG, mouse ascites has roughly 10 mg/ml of IgG
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
Protocol for anti-HA antibody Western Blotting
seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes again(in 1XPBS
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