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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温运输和保存
- 保质期:
一年
- 英文名:
Plant Total Protein Miniprep Kit (2DE)
- 库存:
大量
- 供应商:
上海钰博生物科技有限公司
- 规格:
30次/100次盒
本产本用于快速提取微量植物蛋白,用于双向凝胶电泳分析分析。本产品的其 主要特点是:
1. 可以处理各种植物材料,包括新鲜或冻干的材料。
2. 操作简单。
3. 能有效去除植物样品中常见的污染。
4. 得到的提取物可以直接用于2DE分析。
植物总蛋白质微量提取试剂 (2DE)-Plant Total Protein Miniprep Kit (2DE)使用及效果
将200 mg新鲜的植物材料研磨成粉后加1 mL溶液A,继续研磨成糊状,4℃ 15000 g离心15分钟,上清中加100 μL溶液B,-20℃放至少15分钟,4℃ 15000 g离心15分钟,用1 mL溶液C洗涤沉淀两次,1 mL 溶液D洗涤沉淀一次,晾干待 用。(使用时需加入自备的200 μL 2DE上样液溶解蛋白质沉淀)。
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文献和实验Our protein extraction protocol for two-dimensional gel electrophoresis (2DE) was updated to meet current needs in the field of proteomics. This protocol summarizes our experience using this method since its introduction over 30 years ago
Creating 2DE Databases for the World Wide Web
With the development of the Internet, a growing number of two-dimensional electrophoresis (2DE) databases have become available (60 in 2009, for a total of 425 image maps). By linking the two components constituting 2DE databases, gel images
Shotgun Protein Analysis by Liquid Chromatography-Tandem Mass Spectrometry
Two-dimensional electrophoresis (2DE) is an excellent technology for the analysis of complex protein mixtures, but it has drawbacks, such as hardly detecting very hydrophobic proteins. Shotgun protein analysis is one of the major technologies
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