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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
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- 英文名:
COMPtrol Gt anti-Ms Ig(H&L) Particles Kit
- 库存:
5.0-5.9 um,
- 供应商:
上海熹垣生物
- CAS号:
N/A
- 规格:
3x5 mL
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文献和实验Southern Blotting(Fred Hutchinson Cancer Research Cente
#2: 2% Blocking Reagent in Buffer #1 Heat to 50-70℃; prepare ~60' in advance; sol'n remains turbid Store at 4℃. Add NaAzide for long term storage. Buffer #3: 100mM Tris-Cl, pH 9.5 100mM NaCl 50mM MgCl2 Antibody: 10 %26micro;l anti-digoxigenin-AP Fab
(5 µL DNA, + 1 µL enzyme, + 1.5 µL 10x buffer, + 7.5 µL H2 O) 37ºC x 1-2 hrs. Run on 0.8% agarose gel (in 0.5x TBE gel + 0.5 µg/mL ethidium Br) x 2 hrs, to check DNA quality. B. Cells: Pellet cells and wash 1x in PBS. Optional:
【转贴】immunofluorescence staining
m l buffer for 24 well trays, 1 ml for 6 well trays, etc.) Remove formaldehyde fix, discard in toxic waste bottle, and wash X 3 with PEM buffer, about 2-5 min. per wash.Incubate 5-30 min. with PEM + 0.5% Triton X-100. This step permeablizes the cells
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