[ PROPERTIES ]
Residues: Phe2240~Thr2492
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q9Y4G6
Host: E. coli
Subcellular Location: Cell junction, focal adhesion.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. Predicted isoelectric point: 5.2 Predicted
Molecular Mass: 30.1kDa
Accurate Molecular Mass: 35kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP. (May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc