Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPDGF-BB. Anti-Human PDGF-BB specific antibody was purified by affinity chromatography employing immobilized hPDGF-BB matrix.
Catalog #:
500-P47
Source :
Rabbits
Formulation :
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Stability :
The lyophilized antibody is stable for at least 2 years from date of receipt at -200 C. The reconstituted antibody is stable for at least two weeks at 2-80 C. Frozen aliquots are stable for at least 6 months when stored at -200 C. Avoid repeated freeze-thaw cycles.
Reconstitution:
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
To yield one-half maximal inhibition [ND50 ] of the biological activity of hPDGF-BB (2.5 ng/ml), a concentration of 0.03-0.04 µg/ml of this antibody is required.
Elisa
Indirect:To detect hPDGF-BB by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-BB. Sandwich:To detect hPDGF-BB by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ’s Biotinylated Anti-Human PDGF-BB (500-P47Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-BB.
Western Blot
To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Country of Origin :
USA
Size A
50µg
$195
Size B
100µg
$250
Size C
1mg
$2100
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Usage: For Research Use Only. Not for use in diagnostic or therapeutic procedures.