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- 详细信息
- 文献和实验
- 技术资料
- 目录编号:
500-P54bt
- 库存:
大量
- 宿主:
兔
- 抗体英文名:
Rabbit Biotinylated Anti-Murine IL-4 Polyclonal Antibody
- 是否单克隆:
2
- 保存条件:
-20oC
- 标记物:
生物素标记
- 供应商:
PeproTech(派普泰克)
- 抗原:
IL-4
- 应用范围:
ELISA/WB/IHC/中和(详见说明书)
- 适应物种:
小鼠
- 级别:
RUO
- 保质期:
6个月以上
- 抗原来源:
小鼠
| Preparation : |
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-4. Anti-Murine IL-4 specific antibody was purified by affinity chromatography and then biotinylated. |
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| Catalog #: |
500-P54Bt |
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| Source : |
Rabbits |
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| Formulation : |
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
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| Stability : |
The lyophilized antibody is stable for at least 2 years from date of receipt at -200 C. The reconstituted antibody is stable for at least two weeks at 2-80 C. Frozen aliquots are stable for at least 6 months when stored at -200 C. Avoid repeated freeze-thaw cycles. |
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| Reconstitution: |
Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. |
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| Applications : |
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| Elisa |
Direct :To detect mIL-4 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIL-4. |
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| Western Blot |
To detect mIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
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| Country of Origin : |
USA |
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