PeproTech大鼠抗小鼠 EGF单抗 500ug

Preparation :

Produced in Sprague Dawley rats using highly pure (>98%) recombinant murine EGF as the immunizing antigen. This IgG2_AK antibody was purified from cell culture by Protein G affinity chromatography.

Catalog #:

500-M136

Source :

Rats

Formulation :

A sterile filtered antibody solution was lyophilized from PBS.

Stability :

The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20^o C. Frozen aliquots are stable for at least six weeks at 2-8^o C or for at least six months at -20^o C. Avoid repeated freeze-thaw cycles.

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml.

Applications :

Neutralization

To yield one-half maximal inhibition [ND ₅₀ ] of the biological activity of mEGF (0.1 ng/ml), a concentration of 1.0-1.5 μg/ml of this antibody is required.

Elisa

In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 100 pg/ml of recombinant murine EGF when used with 's biotinylated antigen affinity purified anti-murine EGF (500-P174GBt) as the detection antibody at a concentration of approximately 1.0-2.0 µg/ml.

Western Blot

To detect mEGF by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEGF is 0.25-0.50 ng/lane under non-reducing conditions and 10.0-15.0 ng/lane under reducing conditions.

Country of Origin :

USA

Special Sizes:
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Usage:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.