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- PeproTech
- 400-16L
- 美国
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
PeproTech(派普泰克)
- 库存：
大量
- 英文名：
Recombinant Rat IL-13 (Long Form)
| Synonyms : |
NC300 (Human) P600 (Murine) |
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| Description : |
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1β, TNF-α, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human and murine IL-13 is cross-species reactive. Recombinant rat IL-13 is a 12.3 kDa protein consisting of 113 amino acid residues. |
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| Catalog #: |
400-16L |
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| Source : |
E.coli |
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| Purity : |
Greater than 98% by SDS-PAGE gel and HPLC analyses. |
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| Endotoxin Level : |
Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
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| Biological Activity : |
The ED 50 was determined by the dose-dependent proliferation of TF-1 cells is < 5.0 ng/ml, corresponding to a specific activity of > 2 x 105 units/mg. |
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| AA Sequence : |
TPGPVRRSTS PPVALRELIE ELSNITQDQK TSLCNSSIVW SVDLTAGGFC AALESLTNIS SCNAIHRTQR ILNGLCNQKA SDVASSPPDT KIEVAQFISK LLNYSKQLFR YGH |
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| PubMed Link : |
IL-13+AND+rat |
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| Country of Origin : |
USA |
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文献和实验为纵坐标，在坐标纸上作图，画出标准曲线。 3. 根据样品 OD 值在该曲线图上查出相应 IL-13 含量。 试剂盒性能 1. 灵敏度 ： 最小的 IL-13 检测浓度小于 15pg/ml 。 2. 特异性： 可同时检测重组或天然的大鼠 IL-13 。 不与 大鼠其它细胞因子有交叉反应。 3. 重复性：板内、板见变异系数均小于10.6 ％。 注意事项
Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period
and on prolonged storage of whole blood at -20 and -80ºC, DNA yield was considerably decreased which was probably due to degeneration of the white blood cells in the storage of long period. We have now modified the method for rapid isolation of DNA from blood
Long PCR Reagents and Guidelines
be made using 500 mM Mg(OAc)2, 1 M Tricine and 1 M KOAc stock solutions as follows: 5X Long PCR Buffer 425 mM KOAc 125 mM Tricine pH 8.7 (adjust pH of Tricine stock solution with KOH) 40% glycerol 5% DMSO 1.2 mM
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