- 询价
- PeproTech
- 350-10
- 美国
- 2025年12月25日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
PeproTech(派普泰克)
- 英文名：
Recombinant Human IGF-Binding Protein 1
| Synonyms : |
Insulin-like Growth Factor-Binding Protein 1, IBP-1, Placenta Protein 12 (PP12) |
||||||||||||||||||||||||
| Description : |
IGF-BPs controls the distribution, function and activity of IGFs in various cell tissues and body fluids. Currently there are seven named IGF-BPs that form high affinity complexes with both IGF-I and IGF-II. IGF-BP1 is a 25.4 kDa cysteine-rich secreted protein expressed in liver, deciduas, and kidneys and is the most abundant IGF-BP in amniotic fluid. Levels of IGF-BP1 in serum are lowest after food. IGF-BP1 binds to both IGF-I and IGF-II with equal affinity. Phosphorylated IGF-BP1 hinders IGF actions, where as nonphosphorylated IGF-BP1 is stimulatory. Recombinant human IGF-BP1 is a 25.4 kDa protein consisting of 235 amino acid residues (Isoform A). |
||||||||||||||||||||||||
| Catalog #: |
350-10 |
||||||||||||||||||||||||
| Source : |
E.coli |
||||||||||||||||||||||||
| Purity : |
Greater than 98% by SDS-PAGE gel and HPLC analyses |
||||||||||||||||||||||||
| Endotoxin Level : |
Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
||||||||||||||||||||||||
| Biological Activity : |
The ED 50 was determined by its ability to inhibit IGF-I induced proliferation of MCF-7 is ≤ 0.5 μg/ml in the presence of 6 ng/ml of human IGF-I. |
||||||||||||||||||||||||
| AA Sequence : |
MAPWQCAPCS AEKLALCPPV SASCSEVTRS AGCGCCPMCA LPLGAACGVA TARCARGLSC RALPGEQQPL HALTRGQGAC VQESDASAPH AAEAGSPESP ESTEITEEEL LDNFHLMAPS EEDHSILWDA ISTYDGSKAL HVTNIKKWKE PCRIELYRVV ESLAKAQETS GEEISKFYLP NCNKNGFYHS RQCETSMDGE AGLCWCVYPW NGKRIPGSPE IRGDPNCQIY FNVQN |
||||||||||||||||||||||||
| PubMed Link : |
IGF+AND+Binding+AND+Protein+AND+1 |
||||||||||||||||||||||||
| Country of Origin : |
USA |
||||||||||||||||||||||||
|
|
|||||||||||||||||||||||||
|
|
|
||||||||||||||||||||||||
|
|
|
||||||||||||||||||||||||
|
|
Special Sizes: |
||||||||||||||||||||||||
|
|
Usage: |
||||||||||||||||||||||||
PeproTech重组人IGF-BP1参考文献链接：
http://www.peprotech.com/en-US/Pages/References/350-10
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验我们以Recombinant Human IL-4 (重组 人IL-4，产品编号：200-04)的说明书为例，对细胞因子或蛋白的溶解方法进行详细的阐述。 拿到重组 人IL-4的说明书后，您会发现有一段关于Reconstitution(重悬)的叙述，这段内容含有溶解相关的所有信息。 1. Centrifuge the vial prior to opening 第 1 步：开盖前离心试剂管 PeproTech的细胞因子 或蛋白冻干粉装盛在塑料管中，为无菌包装。冻干
大家知道，PeproTech的所有细胞因子和蛋白均为冻干粉，这使得运输非常便捷，只要常温即可。而且，细胞因子和蛋白冻干粉非常稳定，在-20o C或-80o C条件下可保存数年。冻干粉在使用前需进行溶解，然后以液体形式加到培养体系或注射入动物体内。溶解步骤非常关键，因溶解不好会导致细胞因子或蛋白的失活，这也是很多用户在实际使用中经常遇到的问题。 应该如何进行正确的溶解呢？ 下面我们以Recombinant Human IL-4 (重组人IL-4，产品编号：200-04
Plasmid Preparation on Sephacryl S1000
-containing bacteria, (2 ) lysis of bacteria, (3 ) low-speed centrifugation to remove the majority of bacterial DNA and protein, (4) an ultracentrifugation step in cesium chloride and ethidium bromide to separate plasmid DNA from RNA and chromosomal DNA
技术资料暂无技术资料 索取技术资料
