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- PeproTech
- 120-15
- 美国
- 2025年11月05日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
PeproTech(派普泰克)
- 库存：
大量
- 英文名：
Recombinant Human Activin B
| Synonyms : |
Activin beta-B, Inhibin beta-2 |
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| Description : |
Activin B is a TGF-β family member that exhibits a wide range of biological activities including regulation of embryogenesis, osteogenesis, hematopoiesis, reproductive physiology and hormone secretion from the hypothalamic, pituitary and gonadal glands. Activin B, like certain other members of the TGF-β family, signals through the ActRII receptor (Activin Receptor type II). Human Activin B is a 25.6 kDa disulfide-linked homodimer consisting of two βB chains, each containing 115 amino acid residues.*Manufactured using BTI-Tn-5B1-4 cells under license from the Boyce Thompson Institute for Plant Research, Inc |
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| Catalog #: |
120-15 |
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| Source : |
(BTI-Tn-5B1-4) High-5 Insect cells* |
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| Purity : |
Greater than 95% by SDS-PAGE gel and HPLC analyses. |
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| Endotoxin Level : |
Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
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| Biological Activity : |
The ED 50 as determined by its ability to inhibit the proliferation of murine MPC-11 cells is ≤ 2ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg. |
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| AA Sequence : |
GLECDGRTNL CCRQQFFIDF RLIGWNDWII APTGYYGNYC EGSCPAYLAG VPGSASSFHT AVVNQYRMRG LNPGTVNSCC IPTKLSTMSM LYFDDEYNIV KRDVPNMIVE ECGCA |
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| PubMed Link : |
Activin+AND+B+AND+human |
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| Country of Origin : |
USA |
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文献和实验FREEZE-CRACK b-GALACTOSIDASE STAINING PROTOCOL
FREEZE-CRACK b -GALACTOSIDASE STAINING PROTOCOL FIRE LAB 1. Prepare humidity chamber: line large square plate with humid paper towels and place 4 5ml plastic pipets across chamber to support slides. Place
液稀释。 2. 一定要溶解到指定的浓度。 蛋白在一定的浓度范围内可以保持良好的稳定性，这个范围就是说明书上所标明的浓度范围，该例为0.1-1.0 mg/ml。这个浓度范围对于不同的细胞因子或重组蛋白是不同的。如重组人FGF-6(产品编号：100-30)为0.5-1.0 mg/ml，而重组人Activin B(产品编号：120-15)则一定要是0.5 mg/ml。 问：高于或低于该浓度范围会有什么问题呢? 答：首先，细胞因子或蛋白会不稳定，即很容易出现活性
范围，该例为0.1-1.0 mg/ml。这个浓度范围对于不同的细胞因子或重组蛋白是不同的。如重组人FGF-6(产品编号：100-30)为0.5-1.0mg/ml，而重组人Activin B(产品编号：120-15)则一定要是0.5mg/ml。 高于或低于该浓度范围会有什么问题呢？ 答：首先，细胞因子或蛋白会不稳定，即很容易出现活性下降的现象。其次，高于这个浓度范围，可能会超过该蛋白的最大溶解浓度，即蛋白无法完全溶解。再者，高于或低于该浓度范围，蛋白可能会出现聚集(aggregation
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