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- 一基
- 上海
- YJ-172023971
- 2025年07月16日
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RT(常温）
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100
- 供应商：
上海一基实业有限公司
- CAS号：
172023-97-1
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文献和实验Confocal Laser Scanning Microscopy Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos
for this protocol) for general morphological assessment, and second, staining live embryos with the vital stain LysoTracker� Red (LT), followed by fixation and clearing with benzyl alcohol∶benzyl benzoate (BABB) to visualize areas of apoptosis (see Note 1 , ref
minutes. Rinse embryos 2x in Methanol for 5-10 minutes to dehydrate prior to clearing. Clear embryos (if desirable) in 2:1 :: benzyl benzoate:benzyl alcohol. Reagents/Solutions PBS 0.8 NaCl 0.02 KCl 0.02M
Immunofluorescence- Whole Mount
''. **Incomplete dehydration of the embryos will result in the unpleasent disintagration of the embryos when cleared. Clear embryos by removing most of the methanol and adding 0.5mL of benzyl benzoate:benzyl alcohol :: 2:1. Mix gently, when embryos settle
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