Synthetic MAP peptide derived from human beta-Actin
亚型:
IgG
克隆性:
多克隆
宿主:
Rabbit
应用范围:
WB, IHC-P, IF/ICC, P-ELISA
浓度:
1mg/1ml
抗体英文名:
Profilin 2
规格:
50ul/100ul/200ul
规格:
50ul
产品价格:
¥780.0
规格:
100ul
产品价格:
¥1380.0
规格:
200ul
产品价格:
¥2200.0
英文名称TPH
中文名称色氨酸羟化酶抗体
别 名MGC119994; TPH 1; TPH; TPH1; TPRH; Tryptophan 5 hydroxylase 1; Tryptophan 5 monooxygenase 1; Tryptophan 5 monooxygenase; Tryptophan hydroxylase 1; Tryptophan 5-hydroxylase 1; TPH1_HUMAN.
Specific References (1) | bs-1215R has been referenced in 1 publications.[IF=2.75] Dong, Xian-Zhe, et al. "A representative prescription for emotional disease, Ding-Zhi-Xiao-Wan restores 5-HT system deficit through interfering the synthesis and transshipment in chronic mild stress-induced depressive Rats." Journal of Ethnopharmacology (2013). WB ; Rat. PubMed:24184266
规格价格50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价
说 明 书50ul 100ul 200ul
研究领域细胞生物 神经生物学 信号转导
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Horse, Rabbit,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量49kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human TPH:31-150/444
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009].
Subunit:
Homotetramer.
Tissue Specificity:
Isoform 2 seems to be less widely expressed than isoform 1.
Similarity:
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Contains 1 ACT domain.
SWISS:
P17752
Gene ID:
7166
Database links:
Entrez Gene: 7166 Human
Entrez Gene: 21990 Mouse
Entrez Gene: 24848 Rat
Omim: 191060 Human
SwissProt: P17752 Human
SwissProt: P17532 Mouse
SwissProt: P09810 Rat
Unigene: 591999 Human
Unigene: 248684 Mouse
Unigene: 94788 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Sample: Liver (Mouse)Lysate at 40 ug Primary: Anti-TPH(bs-1215R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49kD
Sample: Lung (Mouse)Lysate at 40 ug Primary: Anti-TPH(bs-1215R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49kD
Sample: HL60/HepG2/K562 Cell Lysate at 40 ug Primary: Anti- TPH(bs-1215R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 53kD
Sample: HL60/HepG2/K562 Cell Lysate at 40 ug Primary: Anti- TPH(bs-1215R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 54 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TPH) Polyclonal Antibody, Unconjugated (bs-1215R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.