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- 2025年09月16日
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- 文献和实验
- 技术资料
- 库存：
大量
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长期储存.低温.蔽光
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出厂后两年
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西宝生物
- 英文名：
TAMA PURE HNO3 55% AA-100
- 规格：
分析试剂
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文献和实验, or equivalent). Adjust the pH of the solution to 6.7 with 5 M KOH, and then add pure H2 O to bring the final volume to 100 ml. Sterilize the solution by filtration through a disposable prerinsed Nalgene filter (0.45-mm pore size). Divide into aliquots and store
) containing 4mM L-glutamate, 4.5 mg/ml glucose and 0.11 mg/ml sodium pyruvate (Invitrogen UK, Cat no: 41966-029), supplemented with 100 U/ml Penicilin and 100 µg/ml Streptomycin (1% v/v Penicilin/Streptomycin solution, Invitrogen UK, Cat no: 15140-122
Screening of cDNA and genomic libraries
you are lucky. If not start over again, pick the positive clones but this time make sure you plate out less phage on the plate. You should try to plate out less than 100 pfu on the tertiary plates. When you have picked a plaque pure clone from a lambda
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