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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Room Temperature
- 保质期:
1年
- 英文名:
HEPES
- 库存:
10瓶
- 供应商:
朗智(上海)生物科技有限公司
Description
Gibco® HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10–25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incubator. HEPES is a good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stable, and has very low visible and UV light absorbance.Dual-site cGMP Manufacturing and Quality System
Gibco® HEPES is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer this identical Gibco® HEPES product made in our Grand Island facility (15630-080). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Specifications
| Form: | Liquid |
|---|---|
| Green Features: | Sustainable packaging |
| Product Size: | 100 mL |
| Shipping Condition: | Room Temperature |
| pH Range: | 7.2 - 7.5 |
Contents & storage
Storage conditions: 2°C to 8°CShipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
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文献和实验化学名:2-[4-(2-Hydroxyethyl)-1-piperazinyl] ethanesulfonic acid中文名:羟乙基哌嗪乙硫磺酸分子式:C8H18N2O4S分子量:238.3HEPES是一种非离子两性缓冲液,其在pH 7.2 - 7.4 范围内具有较好的缓冲能力。其最大优点是在开放式培养或细胞观察时能维持较恒定的PH值。在这种培养条件下,细胞培养瓶的盖子应拧紧,以防止培养液中所需的少量碳酸盐散入空气中。大多数培养液中含有酚红作为pH指示剂,酸性培养液呈橙黄色,碱性培养液呈深红
试剂盒里。对细胞无毒性作用。它是一种氢离子缓冲剂,能较长时间控制恒定的pH范围。使用终浓度为10-50mmol/L。 关于HEPES 缓冲溶液的配制,不同的文献资料有不同的说法。根据用途,一种是单纯的HEPES+NaOH,另一种是HEPES+盐。各种配制方法总结如下: 一、500ml 1 M HEPES, pH = 7.0 Stock solution的配制 119.15 g HEPES 溶解在400ml蒸馏水中,加0.5~1M 的NaOH水溶液调节至少所需pH(HEPES
相关专题 细胞培养基的配制 细胞培养专题 HEPES是一种非离子两性缓冲液 ,其在pH 7.2 - 7.4 范围内具有较好的缓冲能力。其最大优点是在开放式培养或细胞观察时能维持较恒定的PH值.在这种培养条件下,细胞培养瓶的盖子应拧紧,以防止培养液中所需的少量碳酸盐散入空气中.大多数培养液中含有酚红作为pH指示剂,酸性培养液呈橙黄色,碱性培养液呈深红色.HEPES有些昂贵,在高浓度时对一些细胞可能有毒. 原则上,HEPES作为缓冲剂可用
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