- 询价
- 一基
- 上海
- YJ-P191423
- 2025年07月12日
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- 技术资料
- 保存条件：
RT(常温）
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详见标签
- 库存：
100
- 供应商：
上海一基实业有限公司
- CAS号：
148018-33-1
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文献和实验A New Method for Accurately Measuring 1-Pyrroline-5-Carboxylate Synthetase Activity
Proline is a key factor in plant adaptation to environmental stresses. The Δ1 -pyrroline-5-carboxylate synthetase catalyzes the first committed step and the rate-limiting step for proline biosynthesis in both plants and mammals. This enzyme
Phycoerythrin conjugation protocol
DMSO Dimethyl sulphoxide, anhydrous SPDP 3-(2-pyridyldithio)propionic acid N-hydroxysuccinimide ester (Sigma) SMCC Succinimidyl trans-4-(N-maleimidylmethyl)cyclohexane-1-carboxylate
Deproteination using phenol/chloroform
'Phenol' as used is Analar grade. Phenol should be melted at 65℃,8-hydroxyquinoline added to a final concentration of 0.1%, and equilibrated three times with an equal volume of 1M Tris.HCl, pH 7.0. The final Tris wash is replaced with TE (10
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