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1240502-50-4
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文献和实验Purification of TFIIA from E. coli
.Insoluble material is pelleted for 10 minutes at 10K in SS-34 rotor at 4 degrees.Save supernatant. At this point,a gel can be run with induced cells,crude extract,and urea extract.Most of the protein in the urea extract should be the expressed TFIIA subunit
Purification of TFIIA from E. coli
Purification of TFIIA from E.coli Linda Warfield,Hahn Lab,2003 Volumes given are for 2L of cells for each subunit (Toa1 and Toa2). Toa1 and Toa2 subunits are expressed separately in cultures of OD0.6 for 2 hours with 0.4mM IPTG at 37 degrees
Membrane Protein Insertion in E. coli
Integral membrane proteins typically span the lipid bilayer with hydrophobic α helices. These proteins can span the membrane once or multiple times with hydrophilic domains facing both sides of the membrane. In Escherichia coli
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