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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT(常温)
- 保质期:
详见标签
- 库存:
100
- 供应商:
上海一基实业有限公司
- CAS号:
16162-28-0
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文献和实验Mix a 10 ul DNA sample with a 3.2 ul labelling solution gently with pipetting ten times. Incubate at 27oC for 30 min. Add 3 ul Stop solution. Fill the cathodal tank (top) with 300 ml of 1x 1-D buffer. Wash out the 1-D buffer containing 20
for egg plant) sample on a 1-D gel. Run electrophoresis at 130 Volts (120 - 140 Volts) for 40 hrs (until the center of BPB reaches 40 cm and that of XC reaches 19 cm from the top of the gel)(Photo) . F. Preparation of 2-D Gel
RLCS (Restriction Landmark cDNA Scanning) Protocol
of 11(W) x 350(L) x 3(D) mm. Remove any trace of the buffer. Pour 3 ml of 1x HB containing 1000 units of Hinf I and 0.01% BSA (enzyme solution). Seal the tray with Saran Wrap (incubate at 37o C for 2 hrs. Meanwhile, set up the 2-D electrophoresis
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