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- 文献和实验
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- 保存条件:
RT(常温)
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详见标签
- 库存:
100
- 供应商:
上海一基实业有限公司
- CAS号:
21190-16-9
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文献和实验Small scale His-Tag fusion protein purification under denaturative conditions
buffer: 6 to8M Urea , 20mM Tris pH 7.5, 100mM NaCl, and appropriate imidazole concentrations. Limitations Do not exposed Ni matrices to reducing agents as DTT or DTE ( you can use ßME up to 20mM); chelating agents as EDTA and EGTA; NH
Synthesis of 3-Amino-l-CarboxymethyI-Benzodiazepine (BZA) Peptidomimetics
inhibitors of Ras famesyltransferase (FTase) (6 ,7 ). The central pair of amino acids in the CAAX tetrapeptide was replaced with the nonpeptide scaffold 3-methylamino-1-carboxymethyl-2,3-dihydro-5-phenyl-1H-1,4-benzodiazepin-2-one, (N -Me)BZA, shown below.
the selective functionalization of the 4?amino group of the cytosine base at pH 7 or the 2?amino group of the guanine base at pH 9.4 or at pH 7.4 in the presence of NiCl2 . In particular, the functionality?transfer reaction (FTR) under the alkaline conditions
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