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文献和实验The IODO-GEN Method for Labeling and the Use of Retrograde Axonal Transport to Assay Neurotrophins
In order to look at the retrograde transport of neurotrophic factors from the target tissue to the innervating cell body, the neurotrophin needs to be labeled to a high specific activity. This chapter outlines methods used
A Method for Assaying Deubiquitinating Enzymes
residue in the PESTc portion of the fusion protein was almost exclusively radioiodinated under a mild labeling condition, such as using IODO-BEADS, the enzymes could be assayed directly by simple measurement of the radioactivity released into acid soluble
• stabilizer soln (10% sodium thiosulfate+0.1N NaOH) • IODO-BEAD™(PIERCE) • Hamilton microsyringe (model 702, 25µl, needle gauge 22S, point style #2) • desalting column (Bio-rad DG-10 etc.) • column stand For procedure 2 • PBS
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