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- 2025年07月10日
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RT(常温）
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上海一基实业有限公司
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383907-32-2
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文献和实验Porcine brain tubulin preparation
Homogenization buffer (freshly prepared) 1L PM buffer 100 mM MgATP 1L PMG Buffer 100 mM MgGTP 1M dithiothreitol (DTT) Glutamic acid sodium salt 实验设备 Waring Blender Temperature controlled ultracentrifuge
acid (sodium salt) to the tubulin solution and stir slowly until it is dissolved. Disburse the solution into 31.5 ml ultracentrifuge tubes and pair them by weight. Immerse the centrifuge tubes to the level of the liquid within in a 37oC
do not need to code for a chemical shift dimension, allowing 23 Na images to be obtained at high resolutions as compared to MRS. In this chapter the biological significance of sodium in the brain will be discussed, as well as methods for observing it with 23 Na-MRI
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