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- 2025年07月11日
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66033-69-0
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro-3-indolyl-ß-D-galactoside (X-gal, Holt and Sadler, 1958). When ß-gal cleaves the glycosidic linkage in X-gal, a soluble, colorless indoxyl monomer
than 5-bromo-4-chloro-3-indolyl-β-d -galactoside (X-gal). S-gal is advantageous where β-gal activity is limited including early stage mouse embryos. As a result of the increased sensitivity as well as the color compatibility of S-gal, we successfully combined
of the thymidine analog 5-bromo-2′-deoxyuridine (BrdU), which gets incorporated into DNA during the S-phase, has been used to detect proliferating cells in many tissues including brain (1 ). Single-stranded DNA with incorporated BrdU
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