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- 保存条件:
RT(常温)
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详见标签
- 库存:
100
- 供应商:
上海一基实业有限公司
- CAS号:
122450-96-8
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文献和实验Oligonucleotides Containing Degenerate Bases: Synthesis and Uses
containing it above those with mismatches has led to its use first in probes and more recently in primers (2 –6 ). We discuss I later, but here we describe the synthesis of monomers and oligomers containing the pyrimidine, 6H,8H-3,4-dihydropyrimido[4,5-c][l
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
" moiety (5-bromo-4-chloro-indolyl) in both X-gal and X-Phos is the same and thus once cleavage by the respective enzyme has occurred, the dimerization and oxidation reactions lead to the same halogenated indigo compound. B. Nitroblue tetrazolium (NBT
的烘箱中干燥3h,移至干燥器中冷却至室温。按顺序号依次放入称量瓶中称重(记作b)。 3.抽提 将装有样品的滤纸包用长镊子放入抽提筒中,注入一次虹吸量的1.67 倍的无水乙醚,使样品包完全浸没在乙醚中。连接好抽提器各部分,接通冷凝水水流,在恒温水浴中进行抽提,调节水温在70-80℃之间,使冷凝下滴的乙醚成连珠状(120-150 滴/min 或回流7次/h 以上),抽提至抽取筒内的乙醚用滤纸点滴检查无油迹为止(约需6-12h)。抽提完毕后,用长镊子取出滤纸包,在通风处使乙醚挥发(抽提室温以12-25
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