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CHK1 (Phospho-S345) Antibody抗体

,orb393243,biorbyt
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  • ¥1820
  • biorbyt已认证
  • orb393243
  • 英国
  • 2025年12月04日
  • IF, IHC, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      CHK1 (Phospho-S345) Antibody抗体

    • 抗体英文名

      CHK1 (Phospho-S345) Antibody

    • 应用范围

      IF, IHC, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb393243

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原

      KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S345 of human CHK1 protein. The exact sequence is proprietary.

    • 规格

      30 ul

    产品描述:The CHK1 (Phospho-S345) Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S345 of human CHK1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:CHK1; Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1

    免疫原:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S345 of human CHK1 protein. The exact sequence is proprietary.

    应用稀释比例:WB: 1:500:1000, IHC-P: 1:100:200, IF/ICC: 1:100:500

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:1111, 140583, 12649

    UniProt ID:O35280, O14757, Q91ZN7

    Note:For research use only.

    产品细节图片1
    Western blot analysis of CHK1 (Phospho-S345) expression in Hela (A), HGC27 (B), mouse testis (C), mouse kidney (D), rat testis (E) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 56 kD)

    产品细节图片2
    Immunohistochemical analysis of CHK1 (Phospho-S345) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of CHK1 (Phospho-S345) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

    产品细节图片4
    Direct ELISA antibody dose-response curve using Anti-CHK1 (Phospho-S345) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

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    图标文献和实验
    相关实验
    • Methods for Studying Checkpoint Kinases Chk1

      -terminal regulatory domain. Within the regulatory domain there are two residues, Serine-317 (S317) and Serine-345 (S345), which are phosphorylated in active Chk1 molecules, and subsequently dephosphorylated to inactivate Chk1 and allow mitotic entry

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