- ¥1600
- cloud-clone
- 美国
- SEA079Ra
- 2026年02月12日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
ELISA Kit for Interleukin 6 (IL6)
- 库存：
大量现货
- 供应商：
广州柏赛柯生物
- 规格：
48T///96T
ELISA Kit for Interleukin 6 (IL6) 
MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor
具体报价请参考官网：http://www.cloud-clone.us
或咨询以下联系方式：
客服热线：020-39341005
联系客服QQ: 1195082744
- Product No.SEA079Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range7.8-500pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 3.3pg/mL.
- SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 6 (IL6).
No significant cross-reactivity or interference between Interleukin 6 (IL6) and analogues was observed. - ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
- DownloadInstruction Manual
- Format48T96T 96T*5 96T*10 96T*100
- FOBUS$ 448
For negotiated price and more details, please contact local distributors!
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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文献和实验牛白介素 6（IL6）酶联免疫吸附检测试剂盒预期应用本试剂盒运用双抗体夹心 ELISA 法定量测定牛血清、血浆、组织匀浆、细胞裂解液、细胞培养上清液和其他生物液体中 IL6 含量。试剂盒内容试剂名称数量试剂名称数量96孔板(预包被)196孔板覆膜2标准品2标准品稀释液1×20mL检测溶液A1×120μL检测稀释液A1×12mL检测溶液B1×120μL检测稀释液B1×12mLTMB底物1×9mL终止液1×6mL浓洗涤液(30×)1×20mL使用说明书1需自备的设备及试剂1.450±10nm 滤光
相关专题 &NBS p; Interleukin 6 (interferon, beta 2) Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti
抗原（或抗体）的浓度。操作过程中颇为费时费力的是各步之间的洗板过程。虽然目前有自动化洗板仪器商供，但普通实验室通常并不置备。在小规模的实验（如只有一块酶联板）中，手工向各反应孔中加入洗液（含0.05% Tween的PBS缓冲液）尚可行。但如有较大规模（如5-10块酶联板）实验，即使使用多道移液器吸加洗液也很易使人疲劳。本文以使用商业试剂盒检测人IL6和自行检测小鼠血清中抗体为例，介绍ELISA的一种简便操作方法。人IL6检测试剂盒为eBioscience公司（San Diego, CA, USA
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