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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 英文名：
Biotin-16-UTP
- 库存：
现货
- 供应商：
上海善然生物科技有限公司
Other Notes
仅用于生命科学研究。不可用于诊断。
Quality
Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).
Physical form
Biotin-16-UTP, tetralithium salt, 10mM solution
Application
Biotin-labeled RNA can be used as a hybridization probe for:
• Northern blots
• Southern blots
• Plaque or colony lifts
• RNase protection experiments
• In situ hybridization
• Microarray hybridization[1][2]
General description
For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling.[3] Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively. The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by ELISA using a Streptavidin-AP conjugate.
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文献和实验Detection of DNA Sequences Using Biotinylated Probes
can be located by a variety of methods, including incubation with streptavidin-peroxidase conjugate, which will be described here. Fig. 1. Structure of biotin-11-UTP.
Labeling of Double-Stranded DNA Probes with Biotin
Probes labeled with biotin have been used for hybridizations to Southern blots and to chromosomes in situ since the early 1980s (1 ). Nowadays, the labeling of double-stranded DNA probes with biotin is made easy by the availability of labeling
。5'末端标记法5'末端标记法是通过化学方法将地高辛标记于寡核苷酸的5'末端。首先在5'末端连接上一个氨基连接臂残基，将合成的寡核苷酸纯化后，再使DIG-NHS与5'末端的氨基残基发生共价结合，从而形成标记RNA。DIG-RNA5'3'5'3'DIGAnti-DIG-APAP-DIG-RNA5'3'5'3'X光片曝光化学发光信号蓝紫色 生物素 用生物素标记RNA的方法常见的是用生物素与UTP结合形成Biotin-UTP，以Biotin-UTPATP、CTP、GTP为底物通过RNA聚合酶SP
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