OrigamiB(DE3)产品信息

OrigamiB(DE3)产品信息

订购信息

货号	载体名称	出品公司	载体用途	价格	到货周期
HZ0206	OrigamiB(DE3)		配套大肠杆菌	1000	1-2 周

载体描述

Origami B host strains carry the same mutations in trxB and gor as the original Origami strains, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels by adjusting the concentration of IPTG. Thus the Origami B strains combine the desirable characteristics of BL21, Tuner?, and Origami strains in one strain background. The mutations in trxB and gor are selectable on kanamycin and tetracycline, respectively; therefore, these strains cannot be used with plasmids that can only selected with kanamycin or tetracycline. These strains also include the lon and ompT deficiences of BL21, which increase protein stability.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

Genotype: F^- ompT hsdS_B(r_B^- m_B^-) gal dcm lacY1 ahpC (DE3) gor522:: Tn10 trxB (Kan^R, Tet^R)

载体序列

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