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- 保修期:
2年
- 现货状态:
现货库存
- 库存:
20
- 供应商:
朗智(上海)生物科技有限公司
- 规格:
25支
LS Columns are designed for positive selection of cells. They are also suitable for the depletion of strongly magnetically labeled cells.
LS Columns can be used with the following separators:
- MidiMACS™ Separator
- QuadroMACS™ Separator
- VarioMACS™ Separator, in combination with an LS Column Adapter
- SuperMACS™ II Separator
- MultiMACS Cell24 Separator Plus, in combination with the Single Column Adapter
Column capacity
For lymphoid cells from blood, bone marrow or tissue:Up to 108 magnetically labeled cells from up to 2×109 total cells.
For the capacity of cells from other tissues, e.g., neural tissues, please refer to the data sheet provided with the particular MACS Cell Separation Reagent.
For use with the MultiMACS Cell24 Separator Plus:
Up to 108 magnetically labeled cells from up to 1×109 total cells.
Background information
The matrix of the LS Columns is composed of ferromagnetic spheres, which are covered with a cell-friendly coating allowing fast and gentle separation of cells.When placed in the magnetic field of a MACS® Separator, the spheres amplify the magnetic field by 10,000-fold, thus inducing a high gradient within the column. This is crucial for isolation of cells which are only minimally labeled with MACS® MicroBeads, leaving enough epitopes free for concurrent antibody staining. The space between the spheres is several times larger than primary and most cultured cells. This allows the cells to freely flow through the column. Magnetically labeled cells are held in suspension within the column and do not actually “bind” the column matrix. This suspension minimizes stress on the cells and allows for efficient sterile washing by avoiding cell aggregation.LS Columns can be used to separate material less than 30 μm in size. The columns are individually packed in a sterile way.
更多详情请联系:
朗智(上海)生物科技有限公司
http://lzbiotech.com
Tel: +(86)021-54019192
Mobile: +(86) 13564617637(微信同号)
QQ:370323648
Email: songxinwei@lzbiotech.com
地址: 上海市徐汇区中山南二路777弄1号楼2405室
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文献和实验。 二、磁分离柱的选择 下表为德国Miltenyi公司的分离柱种类,其中MS, LS, XS柱适合于正性细胞选择,如果磁标记很强,也可用于负性选择。Large Cell Columns与MS柱相比,孔径较大,适合于人和动物大细胞的正性选择,如巨核细胞。新的LD柱是为最方便的负选设计,代替了AS 和BS柱。LD 柱和CD及D柱即使在磁标记很弱的情况下也能获得最高的负选效率。µ 和M柱
MACS(磁柱分选)生产商:Miltenyi Biotec、stem cell、dynal等
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流式细胞仪、免疫磁珠及亲和板结合分离法分选c kit+肝癌细胞的比较
季青公司;兔抗人c kit多克隆抗体、FITC标记的羊抗兔IgG(1∶200)购自丹麦Santa Cruz公司;抗兔IgG1免疫磁珠购自德国Miltenyi Biotech公司;其他常用试剂为国产分析纯。 1.1.4 主要仪器:免疫磁珠分选磁力架、MS磁性分离柱购自德国Miltenyi Biotec公司;Epics Altra型流式细胞仪购自美国Beckman Coulter公司;各种常用耗材购自广州威佳科技公司。1.2 方法 1.2.1 分离、培养肝癌细胞:参考文献方法,分离
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