pcDNA3.1/V5-His C产品信息

pcDNA3.1/V5-His C产品信息

订购信息

货号	载体名称	出品公司	载体用途	价格	到货周期
HZ0012	pcDNA3.1/V5-His C		哺乳动物表达载体	1000 900	现货

产品参数

载体用途：哺乳动物表达载体
原核抗性：Ampicillin（氨苄青霉素）
真核筛选标记：Neomycin / G418
启动子：CMV promter
标签：C端带V5标签及His标签
表达水平：高
5' 测序引物：CMV-f
3' 测序引物：BGH-r
载体拷贝数：高
载体描述

pcDNA ™ 3.1/V5-His A, B, and C are 5.5 kb vectors derived from pcDNA ™ 3.1(+) and designed for high-level expression, purification, and detection of recombinant proteins in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells.

The vectors contain the following elements:
（1）Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the V5 epitope and a polyhistidine metal-binding peptide
（2）Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
（3）Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7)

The control plasmid, pcDNA ™ 3.1/V5-His/lacZ, contains a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. pcDNA ™ 3.1/V5-His/lacZ is included for use as a positive control for transfection, expression, purification, and detection in the cell line of choice.

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