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- 2025年11月25日
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Rat Cell Motility PCR Array
细胞能动性基因表达PCR芯片可用于分析参与细胞运动的84个关键基因的表达。该芯片包含了生长因子和受体趋化的重要受体基因，参与Rho家族信号通路的粘附分子基因，以及编码各种细胞组件的基因。利用该芯片研究者即能够利用该芯片简单可靠地同时检测细胞能动性相关基因的表达。
Chemotaxis: FGF2, ITGB2, MAPK1 (ERK2), MYH10, MYH9, PLAUR (uPAR), PLD1, PRKCA, RAC2, TGFB1, VEGFA, WASF2, WIPF1.
Receptors: EGFR, IGF1R, ITGA4, ITGB1, ITGB2, ITGB3, MET, PLAUR (uPAR), RHO.
Growth Factors: CSF1 (MCSF), EGF, FGF2, HGF, IGF1, TGFB1, VEGFA.
Rho Family GTPAses: Rho Signaling: ACTR2, ACTR3, ARHGDIA, LIMK1, MSN, MYL9, MYLK, PLCG1, PLD1, PRKCA, PTEN, PTPN1, RHO, RHOA, RHOB, RHOC, RND3, ROCK1, VIM.
Rac Signaling: ACTR2, ACTR3, BAIAP2, CFL1, CRK, PAK1, PAK4, PLD1, PRKCA, RAC1, RAC2, STAT3, WASF1, WASF2, WASL.
Cdc42 Signaling: ACTR2, ACTR3, CDC42, PFN1, WASF1, WASF2, WASL.
Adhesion: Cell-Cell Adhesion: DPP4, EGFR, EZR, ITGA4, ITGB1, ITGB2, MSN, MYH9, ROCK1, TGFB1.
Cell-Matrix Adhesion: ACTN1, ACTN3, CSF1 (MCSF), ILK, ITGB1, ITGB2, ITGB3, MMP14, PTEN, PTK2B, PXN, RASA1, RHOA.
Focal Adhesion: ACTN1, ACTN3, ARHGEF7, BCAR1, CAPN1, CAPN2, CAV1, ENAH, ILK, ITGB1, MYL9, PTK2, PTK2B, PXN, TLN1, VASP, VCL.
Leukocyte Adhesion & Rolling: EZR, ITGA4, ITGB1, ITGB2, MSN, ROCK1.
Integrin-Mediated Signaling: BCAR1, ILK, ITGA4, ITGB1, ITGB2, ITGB3, MYH9, PTK2.
Cellular Projections: Filopodia: BAIAP2, CDC42, DIAPH1, EGFR, ENAH, EZR, MSN, RDX, SVIL, VASP.
Lammelipodia: CTTN, DPP4, EGFR, ENAH, FAP, PIK3CA (p110a), PLD1, PTK2, PXN, RDX, SVIL, VASP, VCL, WASF1, WASF2, WASL.
Stress Fibers: ACTN4, DIAPH1, MYH10, MYH9, RHOA, RHOB, RHOC.
Membrane Blebs: ACTN1, ACTN3, ACTN4, EZR, MYH10, MYH9, MYLK, RND3, ROCK1.
Invasive Projections: ACTR2, ACTR3, ARF6, CDC42, CFL1, CTTN, DPP4, EGF, EZR, FAP, MMP14, MMP2 (Gelatinase A), MMP9 (Gelatinase B), MSN, MYH9, PLAUR (uPAR), RAC2, RASA1, SH3PXD2A, SRC, SVIL, TGFB1, VEGFA, WASL, WIPF1.
Growth Cones: ARHGEF7, CDC42, CFL1, PTK2B.
Membrane Ruffles: ACTR2, ACTR3, ARF6, BAIAP2, BCAR1, CTTN, DIAPH1, EZR, ITGB1, MYH9, RAC1, RAC2, RDX, RHOA, TLN1, WASF2.
Cell Polarity: CDC42, CFL1, EZR, IGF1R, ILK, MYH9.
Proteolysis: AKT1, CAPN1, CAPN2, DPP4, FAP, HGF, MMP14, MMP2 (Gelatinase A), MMP9 (Gelatinase B), MYH9, PLAUR (uPAR), TIMP2.
| Array Map of Gene Expression qPCR Array | ||||||||||||
| Rat Cell Motility | Cat.: DRNE2191S | |||||||||||
| 01 | 02 | 03 | 04 | 05 | 06 | 07 | 08 | 09 | 10 | 11 | 12 | |
| A | Actn1 | Actn3 | Actn4 | Actr2 | Actr3 | Akt1 | Arf6 | Arhgdia | Arhgef7 | Baiap2 | Bcar1 | Capn1 |
| B | Capn2 | Cav1 | Cdc42 | Cfl1 | Crk | Csf1 | Cttn | Diaph1 | Dpp4 | Egf | Egfr | Enah |
| C | Ezr | Fap | Fgf2 | Hgf | Igf1 | Igf1r | Ilk | Iqub | Itga4 | Itgb1 | Itgb2 | Itgb3 |
| D | Limk1 | Mapk1 | Met | Mmp14 | Mmp2 | Mmp9 | Msn | Myh10 | Myh9l1 | Myl9 | Mylk | Pak1 |
| E | Pak4 | Pfn1 | Pik3ca | Plaur | Plcg1 | Pld1 | Prkca | Pten | Ptk2 | Ptk2b | Ptpn1 | Pxn |
| F | Rac1 | Rac2 | Rasa1 | Rdx | Rho | Rhoa | Rhob | Rhoc | Rnd3 | Rock1 | Sh3pxd2a | Src |
| G | Stat3 | Svil | Tgfb1 | Timp2 | Tln1 | Vasp | Vcl | Vegfa | Vim | Wasf1 | Wasf2 | Wipf1 |
| H | Actb | B2m | Hprt1 | Ldha | Rplp1 | RGDC | NTC | NTC | NTC | RIQC | RIQC | RIQC |
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文献和实验Preparation of Rat Liver Cell Cytosol
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Preparation of Rat Liver Cell Cytosol
These protocols should yield enough cytosol and organelles for 1-200 MT/Organelle motility assays. Solutions and Reagents Freshly removed or flash frozen rat liver PBS Homogenization Buffer
Evaluation of Immunogenicity of Rat ES-Cell Derived Endothelial Cells
in this specific area by a set of in vitro test systems using endothelial ES cell derivatives. This chapter describes the strategies and methods used to analyze immunogenicity of rat ES cell derived endothelial cells (RESC) in comparison to adult mature
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