Capan-1 cells typically recover very slowly from cryopreservation, and may need more than a week before the cells attach well and grow sufficiently to be subcultured. Unfortunately this is normal for this cell line. It is normal for cultures of Capan-1 to have many cells which remain in suspension, these cells are viable and should be retained by gentle centrifugation and added back to the adherent population (in the same flask). Do not separate the populations (or discard floating cells) because the culture will become too dilute. Patience is the key to culturing these cells. HTB-79 cells grow as adherent patches with an epithelial-like morphology. Cells on the edges of a patch are somewhat elongated, while cells in the center of the patch are cuboidal and small. Growth will be patchy initially and the cells are very small with epithelial-like morphology. Theese cultures only become 80-90% confluent and will begin to detach at that confluence. There are also normally empty spaces in the culture vessels.